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Transmission Electron Microscopes

The Unit has two transmission electron microscopes available for staff and student use: The FEI F20 CRYO FEGTEM, and the FEI T20 TEM. The instruments can be booked in advance for as long as is required for a particular experiment subject to user demand at that particular time. The Unit offers training in the operation of these instruments.

  • Training

    At present the demand for the use for the use of the TEMs is such that new users are usually instructed individually by their supervisors or by Mohamed Jaffer (who is the EM Officer responsible for TEM applications). If there is demand for it we will certainly develop a systematic course covering various aspects of TEM - It would certainly be beneficial to the graduate students who often leave with very limited insights into the technology that they spend most of their time using. Please e-mail us at trevor.sewell@uct.ac.za or send us an enquiry through our online enquiry form. if you would like such a course to be run and also what specific topics you would like covered.

    You can also browse through our online lectures for the EM for Biologists training course, which covers LM, SEM, TEM and Image Analysis.

  • FEI F20 Cryo TEM

    FEI F20 TEM

    FEI F20 TEM

    In 2007, the Unit took delivery of the FEI Tecnai F20 field emission cryo-TEM – the first instrument in this class in Africa. This instrument enables the determination of the three-dimensional structures of biological objects, including viruses and protein complexes at high resolution.

    • High performance in Cryo FEGTEM imaging 200kV with cryo capabilities
    • Plate camera with plates and desiccators - discontinued
    • Gatan Ultrascan 4000 CCD camera 4096x4096
    • Low dose imaging mode and cryo transfer capability
    • TEM Scripting
    • Cryobox
    • Standard single tilt holderVarious sample holders:
      • Gatan 914 high tilt cryo transfer holder and workstation – tilt range +/-80deg
      • FEI Double tilt holder
      • Fischione (Model 2040) dual axis tomography holder
      • Gatan 626 cryoholder and workstation
      • Single-tilt Gatan 626/60 Cryo-transfer holder and workstation with Smartset controller
    • FEI TECNAI F20 FEGTEM Manual (427 pages, 14.5 MB, PDF)
    • Manufacturers website
  • FEI T20 TEM

    FEI T20 TEM

    FEI T20 TEM

    In 2010, the Unit took delivery of a new FEI/Tecnai T20 TEM with a high resolution camera and EELS system.

    • LaB6 emitter – 200kV
    • Gatan Tridiem 863 UHS 200kV for Energy Filtered microscopy
    • CCD camera embedded (2048X2048)
    • Low dose imaging mode and cryo transfer capabilities
    • TEM Scripting
    • Objective Lens: FEI Super twin
    • Cryobox
    • Standard single tilt holder
    • Various sample holders
      • Gatan 914 high tilt cryo transfer holder and workstation – tilt range +/-80deg
      • FEI Double tilt holder
      • Fischione (Model 2040) dual axis tomography holder
      • Gatan 626 cryoholder and workstation
      • Single-tilt Gatan 626/60 Cryo-transfer holder and workstation with Smartset controller
    • Quick Start Manual (37 pages, 2.67 MB, PDF)
  • Sample Holders applicable to both Tecnai TEM
    • Gatan 914 high tilt cryo transfer holder and workstation – tilt range +/-80deg
    • FEI Double tilt holder
    • Fischione (Model 2040) dual axis tomography holder
    • Gatan 626 cryoholder and workstation
    • Single-tilt Gatan 626/60 Cryo-transfer holder and workstation with Smartset controller
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